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Instructions for using the MQM Mastitis Treatment Decision System
This kit is designed for use on cows that do not have a fever (temp<39.5oC). Severe mastitis cases should be treated according to veterinarian recommended protocols.
Sampling
a) Use sterile technique (Sampling Protocol) and the alcohol swabs provided to collect milk into the empty vial.
b) Open the vial containing 45 ml of diluent and add approximately 5 ml of the mastitis milk (to marked 50 ml line). Close the vial.
c) Label and freeze undiluted sample for submission to diagnostic lab.
Plating diluted samples
a) Gently mix the diluted milk sample and insert the needle of the syringe through the top and draw out 2cc of sample.
b) Remove 1 Red and 1 White Petrifilm and place on a flat level surface. Lift and hold back the plastic film cover.
c) Dispense 1 cc of diluted milk on the centre of each Petrifilm.
d) Replace the plastic film. Place the spreader with the “WHITE” side up gently on the white film. Repeat using the “RED” side on the red film.
Incubation
a) Place the Petrifilms in the incubator at 35 °C. Turbofan Hova-Bator incubator available at enasco.com or farm supply stores.
b) Insure that incubator has a small amount of water for humidity.
c) After 22-24 hours remove the Petrifims for reading.
Download MQM Mastitis Treatment Decision System Instructions.
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